|Portrayed by:||Jamie Luner|
She is a fellow hunter who knew the Winchesters and Bobby Singer, she had been sexually involved with all three of them (With Sam when he had no soul). Annie investigated a haunted house and received a call from a ghost there asking to be freed. So Annie decides to call Sam and Dean in as she heads towards the house. At the house she discovers the bodies of a murdered couple, and moments later is killed herself. Annie becomes a ghost, but is determined to solve the case. With fellow ghost Bobby the two figure out what is going on and help Sam and Dean figure it out with the help of another ghost.
She lingers in the house as a ghost and meets Bobby Singer there as a ghost as well. Later she is given the Hunter's Pyre and is put to rest. While she and Bobby believed that this would send her into oblivion, with the later revelation that ghosts that are put to rest are taken to their true final resting place, she is presumably now in Heaven.