Misha worked in the television series Supernatural, which narrated the life of Sam and Dean Winchester, albeit as fiction. Misha worked together with Jared Padalecki and Jensen Ackles, who respectively were the actors for Sam and Dean. Like all members of the cast and staff, he referred to Sam and Dean as Jared and Jensen. Misha shrugged off the brothers' actions and behaviors as a form of prank and/or effect of illicit drugs.
Eventually, Misha was killed by Virgil, who was actively working on a method to return to the original reality. Misha's blood was used by Virgil to create a Goblet of Blood in order to contact the archangel Raphael.
- Like most of the characters seen on the episode The French Mistake, Misha Collins is based on the eponymous real-life actor playing Castiel in Supernatural.
- The tweets the character sent during the episodes were actually sent in real life by the real actor.
- Misha Collins in real life said in A Very Special Supernatural Special that he wanted the version of himself to be as much of a dick as possible.